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46-272 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-272 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

RASIP1 Antibody

CATALOG NUMBER: 46-272

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CANYRHGPPVATSP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RASIP1
Additional Names:
RASIP1, Ras-interacting protein 1, RAIN, FLJ20401, Ras interacting protein 1
Protein Accession Number:
NP_060275.2
Protein Gi Number:
38570105
Ncbi Gene Id Number:
54922
Background Reference 1:
Natalia Y. Mitin, Melissa B. Ramocki, Alfred J. Zullo, Channing J. Der, Stephen F. Konieczny, and Elizabeth J. Taparowsky Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization J. Biol. Chem. 2004;279 22353-22361

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-272

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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