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Recombinant Rat CD47 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD172a (Catalog: 85-062) at 2 μg/mL (100 μL/well) can bind Human CD47 (Catalog: 85-692) with a linear range of 19.5-3491.5ng/mL.
Recombinant Rat CD47 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Rat CD47 Recombinant Protein (85-692)

CATALOG NUMBER: 85-692

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Rat
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 19 - Lys 140
Fusion Tag:
C-hFc
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
39.72 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human CD172a at 2 μg/mL (100 μL/well) can bind Human CD47 with a linear range of 19.5-3491.5ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Itgp; CD47
Protein Accession Number:
NP_062068.1
Ncbi Gene Id Number:
29364
Background:
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-692

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $112.00 through $280.00

Datasheet
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