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Rat Erythropoietin/EPO Recombinant Protein (85-852)

CATALOG NUMBER: 85-852

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Rat
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala27-Arg192
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.36 kDa
Biological Activity:
Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.7-2.8 ng/mL, corresponding to a specific activity of 3.57×105~1.43×106 units/mg.
Purity:
≥ 90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Erythropoietin, Epo
Protein Accession Number:
NP_058697.1
Ncbi Gene Id Number:
24335
Background:
Erythropoietin (EPO), originally identified for its critical hormonal role in promoting erythrocyte survival and differentiation, is a member of the large and diverse cytokine superfamily. EPO and EPOR function as the primary mediators of a general protective response to tissue hypoxia, which acts to maintain adequate tissue oxygenation through adjustments of circulating red cell mass by using a hormonal feedback-control system involving the kidney and the bone marrow. EPO and EPORs are also expressed by other tissues and organs, including the brain and heart. EPO has also been shown to stimulate mitosis and signaling in astrocytes, endothelial cells, cardiomyoblasts, and cardiomyocytes maintained in vitro

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-852

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
Shipping Info

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