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Recombinant Rat IL-5 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Rat IL-5 stimulates cell proliferation assay using TF-1 Human erythroleukemic cells. The ED<sub>50</sub> for this effect is 0.695-2.78 ng/mL, corresponding to a specific activity of 3.60×10<sup>5</sup>~1.44×10<sup>6</sup> units/mg.
Recombinant Rat IL-5 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Rat IL-5 Recombinant Protein (85-843)

CATALOG NUMBER: 85-843

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
HEK293 cells
Species:
Rat
Source Species:
HEK293 cells
Recombinant Protein Sequence:
Met20-Val132
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
13.91 kDa
Biological Activity:
Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.695-2.78 ng/mL, corresponding to a specific activity of 3.60×105~1.44×106 units/mg.
Purity:
≥ 85% as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Interleukin-5, IL-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, Il5, Il-5
Protein Accession Number:
NP_068606.1
Ncbi Gene Id Number:
24497
Background:
Interleukin-5 (IL-5), which is produced primarily by type 2 T helper lymphocytes (Th2), is an eosinophil differentiation and activation factor. Antagonism of IL-5 activity is being explored as a potential treatment of a number of disease conditions associated with eosinophils in animal models. Interleukin-5 (IL-5) which was previously named T cell replacing factor, B cell growth and differentiation factor, colony forming unit growth stimulating factor and eosinophil differentiation factor is produced predominantly by T cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-843

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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