Coomassie blue staining of rat tissue lysates in 10% SDS-PAGE. Lanes: 1, protein markers; 2, rat rat adrenal; 3, cerebellum; 4, cerebrum; 5, small intestine; 6, skeletal muscle; 7, stomach; 8, testis; and 9, thymus

Rat Normal Tissue Blot II

CATALOG NUMBER: 1582

Tested Applications:

Specifications

Species:

Rat

Samples Loaded:

Lanes: 1, protein markers; 2, rat rat adrenal; 3, cerebellum; 4, cerebrum; 5, small intestine; 6, skeletal muscle; 7, stomach; 8, testis; and 9, thymus

Tested Applications:

Application Note:

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.

Advanced Validation

Properties

Gel Percentage:

Gradient 4-20%

Storage Conditions:

Store at 4°C. The blot can be reused after stripping.

Additional Info

User Note:

Note: Lysate lane order may vary; please consult accompanying shipment paperwork.

Background

Background:

The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The rat tissues included in the blot are adrenal, cerebellum, cerebrum, small intestine, skeletal muscle, stomach, testis, and thymus.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

1582

List Size:

One blot

List Price:

$429.00

Out of stock

Datasheet

Shipping Info

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Rat Normal Tissue Blot II

Tested Applications:

Species:

Samples Loaded:

Tested Applications:

Application Note:

Gel Percentage:

Storage Conditions:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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