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Rat VEGF-A/VEGF164 Recombinant Protein (85-386)

CATALOG NUMBER: 85-386

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Rat
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala27-Arg190
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
20.07 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Rat VEGF164 at 1 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.03-3.6 ng/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.02-0.10 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
MVCD1,VAS,vascular endothelial growth factor A,Vasculotropin,VEGF,VEGFA,VEGF-A,VEGFMGC70609,VPF,VEGFA
Protein Accession Number:
NP_001274037.1
Ncbi Gene Id Number:
83785
Background:
Vascular endothelial growth factor A (VEGFA),also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-386

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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