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Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).
Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).

Rb (phospho Ser795) Antibody

CATALOG NUMBER: 79-162

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of Rb only when phosphorylated at serine 795.
Predicted Molecular Weight:
110 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RB1
Additional Names:
RB, pRb, OSRC, pp110, p105-Rb, ASK1, MAPKKK5, MEKK5, P105-RB, PP105, PP110, RB-1, RB1
Protein Accession Number:
NP_000312.2
Protein Gi Number:
108773787
Ncbi Gene Id Number:
5925
Background:
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Background Reference 1:
Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-162

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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