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Immunofluorescence analysis of U-2 OS cells using Rb antibody (16-832) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Jurkat cells, using Rb antibody (16-832) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using Rb antibody (16-832) at dilution of 1:100. Blue: DAPI for nuclear staining.

RB1 Antibody

CATALOG NUMBER: 16-832

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant protein of human RB1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Jurkat
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RB1
Additional Names:
RB1, RB, pRb, OSRC, pp110, p105-Rb
Ncbi Gene Id Number:
5925
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-832

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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