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Western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstratdetected the Rb protein (arrow).
Confocal immunofluorescent analysis of RB1 Antibody (S608) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
RB1 Antibody (S608) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RB1 Antibody (S608) for immunohistochemistry.
Western blot analysis in Daudi,Raji cell line lysates (35ug/lane).This demonstratdetected the Rb protein (arrow).

RB1 Antibody

CATALOG NUMBER: 62-205

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from human RB1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
106 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RB1
Additional Names:
Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Protein Accession Number:
P06400
Protein Gi Number:
132164
Ncbi Gene Id Number:
5925
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer.[1] One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins.[2][3] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.
Background Reference 1:
Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004).
Background Reference 2:
Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004).
Background Reference 3:
Borah, S., et al., J. Virol. 78(19):10336-10347 (2004).
Background Reference 4:
Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-205

List Size:
400 ul

List Price:

$495.00

Datasheet
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