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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Antibody used in WB on Human MCF7 at 1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

RBBP5 Antibody

CATALOG NUMBER: 25-189

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBBP5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RBBP5 antibody can be used for detection of RBBP5 by ELISA at 1:12500. RBBP5 antibody can be used for detection of RBBP5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
59 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RBBP5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RBBP5
Additional Names:
RBBP5, RBQ3, SWD1
Protein Accession Number:
NP_005048
Protein Gi Number:
53759148
Ncbi Gene Id Number:
5929
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RBBP5 contains 6 WD repeats. It binds preferentially to underphosphorylated retinoblastoma protein. The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.
Background Reference 1:
Olsen, J.V., (2006) Cell 127 (3), 635-648.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-189

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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