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Western blot analysis of extracts of various cell lines, using RBBP7 antibody (22-594) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of MCF-7 cells using RBBP7 antibody (22-594). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug RBBP7 antibody (22-594). Western blot was performed from the immunoprecipitate using RBBP7 antibody (22-594) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody (22-594) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using RBBP7 antibody (22-594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

RBBP7 Antibody

CATALOG NUMBER: 22-594

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
ChIP: 1:50 - 1:200
Positive Control 1:
SW480
Positive Control 2:
HT-29
Positive Control 3:
MCF-7
Positive Control 4:
293T
Positive Control 5:
Mouse spleen
Positive Control 6:
Mouse ovary
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RBBP7
Additional Names:
Histone-binding protein RBBP7, Histone acetyltransferase type B subunit 2, Nucleosome-remodeling factor subunit RBAP46, Retinoblastoma-binding protein 7, RBBP-7, Retinoblastoma-binding protein p46, RBBP7, RBAP46
Ncbi Gene Id Number:
5931
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-594

List Size:
50 uL

List Price:

$285.00

Out of stock

Datasheet
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