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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

RBL1 Antibody

CATALOG NUMBER: 60-514

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1007-1035 amino acids from the C-terminal region of human RBL1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
121 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RBL1
Additional Names:
Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, p107, pRb1, RBL1
Protein Accession Number:
P28749
Protein Gi Number:
90103515
Ncbi Gene Id Number:
5933
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is similar in sequenceand possibly function to the product of the retinoblastoma 1 (RB1)gene. The RB1 gene product is a tumor suppressor protein thatappears to be involved in cell cycle regulation, as it isphosphorylated in the S to M phase transition and isdephosphorylated in the G1 phase of the cell cycle. Both the RB1protein and the product of this gene can form a complex withadenovirus E1A protein and SV40 large T-antigen, with the SV40large T-antigen binding only to the unphosphorylated form of eachprotein. In addition, both proteins can inhibit the transcriptionof cell cycle genes containing E2F binding sites in theirpromoters. Due to the sequence and biochemical similarities withthe RB1 protein, it is thought that the protein encoded by thisgene may also be a tumor suppressor. Two transcript variantsencoding different isoforms have been found for this gene.
Background Reference 1:
Jayadeva, G., et al. J. Biol. Chem. 285(39):29863-29873(2010)
Background Reference 2:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-514

List Size:
400 ul

List Price:

$495.00

Datasheet
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