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Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

RBL2 Antibody

CATALOG NUMBER: 56-286

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This RBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human RBL2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:2000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
128 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RBL2
Additional Names:
Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2, RBL2, RB2
Protein Accession Number:
Q08999
Protein Gi Number:
116242746
Ncbi Gene Id Number:
5934
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RBL2 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. RBL2 may act as a tumor suppressor.
Background Reference 1:
Schaffer, B.E., et al. Cancer Res. 70(10):3877-3883(2010)
Background Reference 2:
Barrow-Laing, L., et al. Virology 400(2):233-239(2010)
Background Reference 3:
Lu, F., et al. J. Virol. 84(6):2697-2706(2010)
Background Reference 4:
Jowett, J.B., et al. Diabetes 59(3):726-732(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-286

List Size:
400 ul

List Price:

$495.00

Datasheet
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