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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
RBM12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

RBM12 Antibody

CATALOG NUMBER: 57-966

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This RBM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the Central region of human RBM12.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RBM12
Additional Names:
RNA-binding protein 12, RNA-binding motif protein 12, SH3/WW domain anchor protein in the nucleus, SWAN, RBM12, KIAA0765
Protein Accession Number:
Q9NTZ6
Protein Gi Number:
30173387
Ncbi Gene Id Number:
10137
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that contains severalRNA-binding motifs, potential transmembrane domains, andproline-rich regions. This gene and the gene for copine I overlapat map location 20q11.21. Alternative splicing in the 5' UTRresults in four transcript variants. All variants encode the sameprotein.
Background Reference 1:
Yang, W., et al. BMC Genomics 9, 456 (2008) :
Background Reference 2:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-966

List Size:
400 ul

List Price:

$495.00

Datasheet
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