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Western blot analysis of extracts of various cell lines, using RBM17 antibody (19-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RBM17 antibody (19-006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

RBM17 Antibody

CATALOG NUMBER: 19-006

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
A-549
Positive Control 3:
HeLa
Positive Control 4:
DU145
Positive Control 5:
Rat thymus
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RBM17
Additional Names:
SPF45, splicing factor 45, 45 kDa-splicing factor, splicing factor 45kDa
Ncbi Gene Id Number:
84991
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-006

List Size:
100 uL

List Price:

$436.00

Datasheet
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