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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of RBM3 antibody in NIH-3T3 cell line lysates (35ug/lane)
RBM3 Antibody (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
RBM3 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of RBM3 using rabbit polyclonal RBM3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

RBM3 Antibody

CATALOG NUMBER: 62-881

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This RBM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human RBM3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RBM3
Additional Names:
Putative RNA-binding protein 3, RNA-binding motif protein 3, RNPL, RBM3, RNPL
Protein Accession Number:
P98179
Protein Gi Number:
1710620
Ncbi Gene Id Number:
5935
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.
Background Reference 1:
Zeng,Y., J. Cell. Biochem. 107 (1), 179-188 (2009)
Background Reference 2:
Sureban,S.M., Oncogene 27 (33), 4544-4556 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-881

List Size:
400 ul

List Price:

$495.00

Datasheet
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