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Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of RBM35A in human colon tissue with RBM35A antibody at 2.5 μg/mL.
Immunofluorescence of RBM35A in human colon tissue cells with RBM35A antibody at 20 μg/mL.
Western blot analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

RBM35A Antibody

CATALOG NUMBER: 6185

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RBM35A antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A.
The immunogen is located within amino acids 30 - 80 of RBM35A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
Positive Control 1:
Cat. No. 1472 - Rat Colon Tissue Lysate
Purification:
RBM35A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RBM35A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RBM35A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ESRP1
Additional Names:
RBM35A Antibody: RBM35A, RMB35A, RBM35A, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A
Protein Accession Number:
NP_001030087
Protein Gi Number:
56790297
Ncbi Gene Id Number:
54845
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RBM35A Antibody: RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.
Background Reference 1:
Warzecha CC, Jiang P, Amirikian K, et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J. 2010; 29:3286-300.
Background Reference 2:
Warzecha CC, Shen S, Xing Y, et al. The epithelial splicing factors ESRP1 and ESRP2 positively and negatively regulate diverse types of alternative splicing events. RNA Biol. 2009; 6:546-62.
Background Reference 3:
Leontieva OV and Ionov Y. RNA-binding motif protein 35A is a novel tumor suppressor for colorectal cancer. Cell Cycle 2009; 8:490-7.
Background Reference 4:
Leontieva OV and Ionov Y. RNA-binding motif protein 35A is a novel tumor suppressor for colorectal cancer. Cell Cycle 2009; 8:490-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6185

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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