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Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.
Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 μg/mL.
Immunofluorescence of RBM35B in human lung tissue cells with RBM35B antibody at 20 μg/mL.
Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.
Trial Size

RBM35B Antibody

CATALOG NUMBER: 6187

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (80%)
Immunogen:
RBM35B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B.
The immunogen is located within the last 50 amino acids of RBM35B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RBM35B antibody can be used for detection of RBM35B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
RBM35B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RBM35B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RBM35B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ESRP2
Additional Names:
RBM35B Antibody: RBM35B, RBM35B, PP7059, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B
Protein Accession Number:
NP_079215
Protein Gi Number:
45935393
Ncbi Gene Id Number:
80004
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RBM35B Antibody: RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.
Background Reference 1:
Warzecha CC, Sato TK, Nabet B, et al. ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing. Mol Cell. 2009; 33:591-601.
Background Reference 2:
Warzecha CC, Jiang P, Amirikian K, et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J. 2010; 29:3286-300.
Background Reference 3:
Warzecha CC, Shen S, Xing Y, et al. The epithelial splicing factors ESRP1 and ESRP2 positively and negatively regulate diverse types of alternative splicing events. RNA Biol. 2009; 6:546-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6187

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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