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Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 antibody at 1 μg/mL
Immunohistochemistry of RC3H1 in human small intestine tissue with RC3H1 antibody at 5 μg/ml.
Immunofluorescence of RC3H1 in human small intestine tissue with RC3H1 antibody at 20 μg/ml.
Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 antibody at 1 μg/mL
Trial Size

RC3H1 Antibody

CATALOG NUMBER: 7251

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1.
The immunogen is located within amino acids 1040 - 1090 of RC3H1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
125 kDa
Purification:
RC3H1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RC3H1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RC3H1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RC3H1
Additional Names:
RC3H1 Antibody: RNF198, ROQUIN, KIAA2025, RNF198, Roquin-1, RING finger and C3H zinc finger protein 1, Roquin
Protein Accession Number:
NP_742068
Protein Gi Number:
73695473
Ncbi Gene Id Number:
149041
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RC3H1 Antibody: The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.
Background Reference 1:
Vinuesa CG, Cook MC, Angelucci C, et al. A RING-type ubiquitin ligase family member required to repress follicular helper T cells and autoimmunity. Nature 2005; 435:452-8.
Background Reference 2:
Bertossi A, Aichinger M, Sansonetti P, et al. Loss of Roquin induces immune deregulation but not autoimmunity. J. Exp. Med. 2011; 208:1749-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7251

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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