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43-007 (1ug/ml) staining of Mouse Brain and KO Mouse Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-007 (1ug/ml) staining of Mouse Brain and KO Mouse Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RCAN1 Antibody

CATALOG NUMBER: 43-007

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HIGSSHLAPPNPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band corresponding to isoform 1L observed in Mouse Brain lysates and this band is not present in the KO mouse. Additional bands around 55kDa were consistently observed, however these band were also observed in the KO mice and therefore they represent non-specific signal. We call for caution when used for other assays than Western blot. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA. Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_004405.3; NP_981962.1; NP_981963.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Knockout Mouse Brain Lysate
Predicted Molecular Weight:
Approx 40 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RCAN1
Additional Names:
RCAN1, regulator of calcineurin 1, ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1, Down syndrome candidate region 1, Down syndrome critical region gene 1, OTTHUMP00000108621, OTTHUMP00000108622, OTTHUMP00000214669, OTTHUMP00000214670, calcipressin-1, calcium and oxidant-inducible mRNA, down syndrome critical region protein 1, modulatory calcineurin-interacting protein 1, myocyte-enriched calcineurin-interacting protein 1, near DSCR proline-rich protein
Protein Accession Number:
NP_004405.3,NP_981962.1,NP_981963.1
Protein Gi Number:
33620725
Ncbi Gene Id Number:
1827

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-007

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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