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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis of RCAN1 using rabbit polyclonal RCAN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.
Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in 293 cell line lysates (35ug/lane).

RCAN1 Antibody

CATALOG NUMBER: 58-724

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RCAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human RCAN1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RCAN1
Additional Names:
Calcipressin-1, Adapt78, Down syndrome critical region protein 1, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN1, ADAPT78, CSP1, DSC1, DSCR1
Protein Accession Number:
P53805
Protein Gi Number:
215274235
Ncbi Gene Id Number:
1827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene interacts withcalcineurin A and inhibits calcineurin-dependent signalingpathways, possibly affecting central nervous system development.This gene is located in the minimal candidate region for the Downsyndrome phenotype, and is overexpressed in the brain of Downsyndrome fetuses. Chronic overexpression of this gene may lead toneurofibrillary tangles such as those associated with Alzheimerdisease. Three transcript variants encoding three differentisoforms have been found for this gene.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Tam, G.W., et al. Biochem. Soc. Trans. 38(2):445-451(2010)
Background Reference 3:
Lee, M.Y., et al. Hum. Mol. Genet. 19(3):468-479(2010)
Background Reference 4:
Sales, K.J., et al. Am. J. Pathol. 176(1):435-445(2010)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-724

List Size:
400 ul

List Price:

$495.00

Datasheet
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