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Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.

REC8 Antibody

CATALOG NUMBER: 57-127

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This REC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-222 amino acids from the Central region of human REC8.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
REC8
Additional Names:
Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1
Protein Accession Number:
O95072
Ncbi Gene Id Number:
9985
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the kleisin family of SMC(structural maintenance of chromosome) protein partners. Theprotein localizes to the axial elements of chromosomes duringmeiosis in both oocytes and spermatocytes. In the mouse, thehomologous protein is a key component of the meiotic cohesioncomplex, which regulates sister chromatid cohesion andrecombination between homologous chromosomes. Multiplealternatively spliced variants, encoding the same protein, havebeen found for this gene.
Background Reference 1:
Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
Background Reference 2:
Erenpreisa, J., et al. Exp. Cell Res. 315(15):2593-2603(2009)
Background Reference 3:
Griffin, J., et al. Syst Biol Reprod Med 54(3):163-165(2008)
Background Reference 4:
Xu, H., et al. Dev. Cell 8(6):949-961(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-127

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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