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Western blot analysis of RECK Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with RECK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of RECK Antibody in HepG2 cell line lysates (35ug/lane)

RECK Antibody

CATALOG NUMBER: 63-860

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RECK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-456 amino acids from the Central region of human RECK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
106 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RECK
Additional Names:
Reversion-inducing cysteine-rich protein with Kazal motifs, hRECK, Suppressor of tumorigenicity 15 protein, RECK, ST15
Protein Accession Number:
O95980
Ncbi Gene Id Number:
8434
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RECK is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis.
Background Reference 1:
Du,Y.Y., et.al., World J. Gastroenterol. 16 (7), 904-908 (2010)
Background Reference 2:
Pesta,M., et.al., Anticancer Res. 29 (11), 4535-4539 (2009)
Background Reference 3:
Takahashi,C., et.al., Tanpakushitsu Kakusan Koso 54 (13), 1742-1746 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-860

List Size:
400 ul

List Price:

$495.00

Datasheet
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