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IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.
IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.
Recombinant

Recombinant CD31 Antibody [C31/1395R]

CATALOG NUMBER: 34-062

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Immunofluorescence: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein A affinity
Clonality:
Recombinant Monoclonal
Isotype:
IgG, k
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
PECAM1
Additional Names:
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Ncbi Gene Id Number:
5175
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-062

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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