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IHC testing of FFPE human colon carcinoma and recombinant Ep-CAM antibody (clone EGP40/1556R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
IHC testing of FFPE human colon carcinoma and recombinant Ep-CAM antibody (clone EGP40/1556R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
Recombinant

Recombinant EpCAM Antibody [EGP40/1556R]

CATALOG NUMBER: 34-081

Clonality:
Recombinant Monoclonal
Tested Applications:
IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Human protein was used as the immunogen for the recombinant Ep-CAM antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC-P
Application Note:
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant Ep-CAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificity:
Does not react with mouse or rat
Purification:
Protein A affinity chromatography
Clonality:
Recombinant Monoclonal
Isotype:
IgG, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
EPCAM
Additional Names:
Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Ncbi Gene Id Number:
4072
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-081

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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