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IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone NX2/1422R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone NX2/1422R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Recombinant

Recombinant NKX2.2 Antibody [NX2/1422R]

CATALOG NUMBER: 34-086

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein A affinity chromatography
Clonality:
Recombinant Monoclonal
Isotype:
IgG, k
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
NKX2-2
Additional Names:
NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
Ncbi Gene Id Number:
4821
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-086

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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