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IHC testing of FFPE human pancreas with NKX2.2 antibody.
IHC testing of FFPE human pancreas with NKX2.2 antibody.
Recombinant

Recombinant NKX2.2 Antibody [RMNK2-1]

CATALOG NUMBER: 34-046

Clonality:
Recombinant Monoclonal
Tested Applications:
IF, IHC
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein was used as the immunogen for this rabbit monoclonal NKX2.2 antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC
Application Note:
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Titering of the NKX2.2 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Purification:
Protein A affinity chromatography
Clonality:
Recombinant Monoclonal
Isotype:
IgG, k
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
NKX2-2
Additional Names:
NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
Ncbi Gene Id Number:
4821
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-046

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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