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Western blot analysis of REEP3 in rat heart tissue lysate with REEP3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of REEP3 in mouse heart tissue with REEP3 antibody at 5 μg/mL.
Immunofluorescence of REEP3 in mouse heart tissue with REEP3 antibody at 20 μg/mL.
Immunofluorescence of REEP3 in A431 cells with REEP3 antibody at 20 μg/mL.<br><br>Green: REEP3 Antibody (5573) <br> Blue: DAPI staining
Immunocytochemistry of REEP3 in A431 cells with REEP3 antibody at 5 μg/mL.
Western blot analysis of REEP3 in rat heart tissue lysate with REEP3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

REEP3 Antibody

CATALOG NUMBER: 5573

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
REEP3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human REEP3.
The immunogen is located within the last 50 amino acids of REEP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
REEP3 antibody can be used for detection of REEP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Purification:
REEP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
REEP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
REEP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
REEP3
Additional Names:
REEP3 Antibody: C10orf74, C10orf74, Receptor expression-enhancing protein 3
Protein Accession Number:
NP_001001330
Protein Gi Number:
47679089
Ncbi Gene Id Number:
221035
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
REEP3 Antibody: Mammalian odorant receptors require accessory proteins such as RTP1 and RTP2 for functional cell surface expression. Receptor expression-enhancing protein (REEP) family members are transmembrane proteins which interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. Recently studies have shown other chemosensory receptors such as bitter taste receptors are also influenced by RTP and REEP family members. REEP3 has also been suggested as a novel candidate gene for autism and as a loci influencing the plasma levels of the liver enzyme alkaline phosphatase.
Background Reference 1:
Saito H, Kubota M, Roberts RW, et al. RTP family members induce functional expression of mammalian odorant receptors. Cell2004; 119:679-91.
Background Reference 2:
Behrens M, Bartelt J, Reichling et al. Members of RTP and REEP gene families influence functional bitter taste receptor expression. J. Biol. Chem.2006; 281:20650-9.
Background Reference 3:
Castermans D, Vermeesch JR, Fryns J-P, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Euro. J. Hum. Genet.2007; 15:422-31.
Background Reference 4:
Yuan X, Waterworth D, Perry JR, et al. Population-based genome-wide association studies reveal six loci influencing plasma levels of liver enzymes. Am. J. Hum. Genet.2008; 83:520-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5573

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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