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Western blot analysis of extracts of various cell lines, using REG3G antibody (18-531) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of MCF-7 cells using REG3G antibody (18-531). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using REG3G antibody (18-531) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

REG3G Antibody

CATALOG NUMBER: 18-531

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of mouse REG3G
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:10 - 1:100
Positive Control 1:
BxPC-3
Positive Control 2:
U-87MG
Positive Control 3:
Rat skeletal muscle
Predicted Molecular Weight:
Observed: 19kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
REG3G
Additional Names:
AI449515, REG-3-gamma, reg III-gamma, regenerating islet-derived protein 3-gamma, generating islet-derived, mouse homolog 3 gamma, pancreatitis-associated protein 3, regenerating islet-derived protein III-gamma
Ncbi Gene Id Number:
19695
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-531

List Size:
100 uL

List Price:

$436.00

Datasheet
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