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Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in WB on Human HepG2 at 1 ug/ml.

RELA Antibody

CATALOG NUMBER: 31-180

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RELA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RELA antibody can be used for detection of RELA by ELISA at 1:62500. RELA antibody can be used for detection of RELA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Predicted Molecular Weight:
60 kDa
Purification:
Antibody is purified by Protein A/G affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RELA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RELA
Additional Names:
RELA, p65, NFKB3
Protein Accession Number:
NP_068810
Protein Gi Number:
223468676
Ncbi Gene Id Number:
5970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.
Background Reference 1:
Ruben, S.M., et al., (1991) Science 251 (5000) 1490-1493..

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-180

List Size:
100 ul

List Price:

$473.00

Datasheet
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