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Fluorescent confocal image of U251 cell stained with RELA Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RELA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).RELA immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in Hela,K562,Raji,Ramos cell line lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with RELA Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RELA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).RELA immunoreactivity is localized to Cytoplasm significantly.

RELA Antibody

CATALOG NUMBER: 60-732

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RELA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-195 amino acids from the Central region of human RELA.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RELA
Additional Names:
Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
Protein Accession Number:
Q04206
Protein Gi Number:
62906901
Ncbi Gene Id Number:
5970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form ofNFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB bytrapping it in the cytoplasm. Phosphorylation of serine residues onthe I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM603258) marks them for destruction via the ubiquitination pathway,thereby allowing activation of the NFKB complex. Activated NFKBcomplex translocates into the nucleus and binds DNA atkappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or Gpurine; and Y is a C or T pyrimidine).
Background Reference 1:
Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010)
Background Reference 2:
Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010)
Background Reference 3:
Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010)
Background Reference 4:
Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-732

List Size:
400 ul

List Price:

$495.00

Datasheet
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