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46-278 (1ug/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-278 (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-278 (1ug/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Renalase Antibody

CATALOG NUMBER: 46-278

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VSIDNKKRNI
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001026879.2 and NP_060833.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RNLS
Additional Names:
RNLS, renalase, C10orf59, chromosome 10 open reading frame 59 , HGNC:25641, FLJ11218 , hypothetical protein LOC55328, renalase, FAD-dependent amine oxidase, RENALASE
Protein Accession Number:
NP_001026879.2,NP_060833.1
Protein Gi Number:
72534708
Ncbi Gene Id Number:
55328
Background Reference 1:
Xu J, Li G, Wang P, Velazquez H, Yao X, Li Y, Wu Y, Peixoto A, Crowley S, Desir GV. Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. J Clin Invest. 2005 May 2;115(5):1275-1280. Epub 2005 Apr 7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-278

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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