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46-279 (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-279 (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Renalase Antibody

CATALOG NUMBER: 46-279

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-QINLRDDKWEVSKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Mouse Kidney and Rat Kidney lysates (calculated MW of 37.8kDa according to Human NP_001026879.1 and 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 0.5-1.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001026879.1 and NP_060833.1).
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
C10orf59
Additional Names:
renalase, C10orf59, chromosome 10 open reading frame 59, FLJ11218
Protein Accession Number:
NP_001026879.1,NP_060833.1
Protein Gi Number:
72534708
Ncbi Gene Id Number:
55328
Background Reference 1:
Xu J, Li G, Wang P, Velazquez H, Yao X, Li Y, Wu Y, Peixoto A, Crowley S, Desir GV. Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. J Clin Invest. 2005 May;115(5):1275-80. Epub 2005 Apr 7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-279

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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