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46-280 (2ug/ml) of Mouse Kidney lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
46-280 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-280 (2ug/ml) of Mouse Kidney lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Renin Receptor Antibody

CATALOG NUMBER: 46-280

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-SIIYRMTNQKIRMD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1). An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6(1):9-19, PMID: 27956412.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ATP6AP2
Additional Names:
ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ELDF10, HT028, MGC99577, MRXE, MSTP009, XMRE, ATPase, H+ transporting, lysosomal interacting protein 2, embryonic liver differentiation factor 10
Protein Accession Number:
NP_005756.2
Protein Gi Number:
15011918
Ncbi Gene Id Number:
10159
Background Reference 1:
Ludwig J, Kerscher S, Brandt U, Pfeiffer K, Getlawi F, Apps DK, Schagger H. Identification and characterization of a novel 9.2- kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules. J Biol Chem. 1998 May 1;273(18):10939-47.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-280

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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