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Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody overnight. The membrane was incubated with donkey anti-rabbit antibody for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data courtesy of Dr. Jingqiong Kang, Department of Neurology, Vanderbilt University.
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody overnight. The membrane was incubated with donkey anti-rabbit antibody for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data courtesy of Dr. Jingqiong Kang, Department of Neurology, Vanderbilt University.

RENT1 Antibody

CATALOG NUMBER: 60-296

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the Central region of human RENT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
124 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UPF1
Additional Names:
Regulator of nonsense transcripts 1, 364-, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, UPF1, KIAA0221, RENT1
Protein Accession Number:
Q92900
Protein Gi Number:
17380291
Ncbi Gene Id Number:
5976
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
Background Reference 1:
Ohnishi, T., et al., Mol. Cell 12(5):1187-1200 (2003). Lykke-Andersen, J., Mol. Cell. Biol. 22(23):8114-8121 (2002). Carastro, L.M., et al., Nucleic Acids Res. 30(10):2232-2243 (2002). Mendell, J.T., et al., Science 298(5592):419-422 (2002). Serin, G., et al., Mol. Cell. Biol. 21(1):209-223 (2001).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-296

List Size:
400 ul

List Price:

$495.00

Datasheet
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