Western blot analysis of RUVBL2 using rabbit polyclonal RUVBL2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL2 gene.

Antibody is used in Western blot to detect RUVBL2 in mouse kidney (left) and SK-Br-3 (right) tissue/cell line lysates.

Reptin/TIP49B/RUVB2 Antibody

CATALOG NUMBER: 60-401

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Homology:

Predicted species reactivity based on immunogen sequence: Xenopus, Bovine

Immunogen:

This Reptin/TIP49B/RUVB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-430 amino acids from the C-terminal region of human Reptin/TIP49B/RUVB2.

Conjugate:

Unconjugated

Tested Applications:

Application Note:

For WB starting dilution is: 1:1000

Predicted Molecular Weight:

51 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

RUVBL2

Additional Names:

RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, 51 kDa erythrocyte cytosolic protein, ECP-51, INO80 complex subunit J, Repressing pontin 52, Reptin 52, TIP49b, TIP60-associated protein 54-beta, TAP54-beta, RUVBL2, INO80J, TIP48, TIP49B

Protein Accession Number:

Q9Y230

Protein Gi Number:

28201890

Ncbi Gene Id Number:

10856

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.

Background Reference 1:

Bauer, A., et al., EMBO J. 19(22):6121-6130 (2000).

Background Reference 2:

Parfait, B., et al., Ann. Genet. 43(2):69-74 (2000).

Background Reference 3:

Wood, M.A., et al., Mol. Cell 5(2):321-330 (2000).

Background Reference 4:

Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-401

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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Reptin/TIP49B/RUVB2 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

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