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Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 μg/mL.
Immunohistochemistry of REQUIEM in human kidney tissue with REQUIEM antibody at 5 μg/mL.
Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 μg/mL.
Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 μg/mL.
Trial Size

REQUIEM Antibody

CATALOG NUMBER: 6109

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
REQUIEM antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Requiem.
The immunogen is located within the last 50 amino acids of REQUIEM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
REQUIEM Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
REQUIEM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
REQUIEM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPF2
Additional Names:
REQUIEM Antibody: REQ, UBID4, ubi-d4, BAF45D, REQ, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein
Protein Accession Number:
AAB81203
Protein Gi Number:
5454004
Ncbi Gene Id Number:
5977
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
Background Reference 1:
Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9.
Background Reference 2:
Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5.
Background Reference 3:
Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6109

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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