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To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.

Resistin Antibody

CATALOG NUMBER: XP-5261

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hResistin (human Resistin).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hResistin.
Sandwich:
To detect hResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Resistin (XP-5261Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.
Western Blot:
To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hResistin specific antibody was purified by affinity chromatography employing immobilized hResistin matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Resistin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
RETN
Additional Names:
ADSF, RSTN, XCP1, FIZZ3, RETN1, HXCP1, UNQ407/PRO1199, Resistin, Adipose tissue-specific secretory factor, ADSF
Protein Accession Number:
Q9HD89
Protein Gi Number:
18202962
Ncbi Gene Id Number:
56729
User Note:
Centrifuge vial prior to opening.
Background:
Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5261

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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