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Western blot analysis using RET monoclonal antibody against truncated RET - GST recombinant protein and STYK1 (aa658 - 1063) / hIgGFc transfected CHO - K1 cell lysate.
Western blot analysis using RET monoclonal antibody against truncated RET - GST recombinant protein and STYK1 (aa658 - 1063) / hIgGFc transfected CHO - K1 cell lysate.

RET Antibody [6E4C4]

CATALOG NUMBER: 32-211

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified truncated recombinant RET (aa896-1063)-GST fusion protein is expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
RET monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RET
Additional Names:
CDHF12, CDHR16, PTC, RET51, Cadherin family member 12PTC, MTC1, HSCR1, MEN2A, MEN2B, CDHF12, RET-ELE1
Protein Accession Number:
P07949
Protein Gi Number:
547807
Ncbi Gene Id Number:
5979
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RET (ret proto-oncogene) is a member of the cadherin superfamily and a receptor tyrosine kinase, which are cell-surface molecules that transduce signals for cell growth and differentiation. It can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.
Background Reference 1:
Young HM. Anderson RB. Anderson CR. Auton Neurosci. 2004, May 31,112(1-2):1-14.
Background Reference 2:
Myers SM. Mulligan LM. Cancer Res. 2004, Jul 1,64(13):4453-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-211

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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