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IHC testing of human colon with Retinoblastoma antibody (clone SPM353). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of human colon with Retinoblastoma antibody (clone SPM353). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Retinoblastoma / Rb Antibody [SPM353]

CATALOG NUMBER: 33-784

Clonality:
Monoclonal
Tested Applications:
IHC-P, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, IP, WB
Application Note:
WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Titering of the Retinoblastoma antibody may be required for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
RB1
Additional Names:
Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Ncbi Gene Id Number:
5925
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-784

List Size:
100 ug

List Price:

$626.00

Datasheet
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