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46-281 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
46-281 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

REV1L Antibody

CATALOG NUMBER: 46-281

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VLQQTYGSTLKVT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
REV1
Additional Names:
REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like
Protein Accession Number:
NP_057400.1,NP_001032961.1
Protein Gi Number:
7706681
Ncbi Gene Id Number:
51455
Background Reference 1:
Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. J Biol Chem. 2001 May 4;276(18):15051-8. Epub 2001 Jan 22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-281

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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