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Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.

RFC3 Antibody

CATALOG NUMBER: 30-152

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RFC3 antibody can be used for detection of RFC3 by ELISA at 1:62500. RFC3 antibody can be used for detection of RFC3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
40 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RFC3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RFC3
Additional Names:
RFC3, MGC5276, RFC38
Protein Accession Number:
NP_002906
Protein Gi Number:
4506489
Ncbi Gene Id Number:
5983
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Background Reference 1:
Zou, L., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (24), 13827-13832.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-152

List Size:
100 ul

List Price:

$519.00

Datasheet
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