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Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.
Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 μg/mL.<br><br>Green: RFX1 Antibody (8199) <br> Blue: DAPI staining
Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 μg/mL.
Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

RFX1 Antibody

CATALOG NUMBER: 8199

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%)
Immunogen:
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.
The immunogen is located within the last 50 amino acids of RFX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 108 kDa
Observed: 125 kDa
Purification:
RFX1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RFX1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RFX1
Additional Names:
RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1
Protein Accession Number:
NP_002909
Protein Gi Number:
238859557
Ncbi Gene Id Number:
5989
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).
Background Reference 1:
Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44.
Background Reference 2:
Katan-Khaykovich Y and Shaul Y. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes. J. Biol. Chem. 1998; 273:24504-12.
Background Reference 3:
Hsu YC, Liao WC, Kao CY, et al. Regulation of FGF1 gene promoter through transcription factor RFX1. J. Biol. Chem. 2010; 285:13885-95.
Background Reference 4:
Su JC, Tseng PH, Hsu CY, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget 2014; 5:4909-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8199

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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