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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

RFX3 Antibody

CATALOG NUMBER: 56-549

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This RFX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 661-690 amino acids from the C-terminal region of human RFX3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RFX3
Additional Names:
Transcription factor RFX3, Regulatory factor X 3, RFX3
Protein Accession Number:
P48380
Protein Gi Number:
32172437
Ncbi Gene Id Number:
5991
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the regulatory factor X genefamily, which encodes transcription factors that contain ahighly-conserved winged helix DNA binding domain. The proteinencoded by this gene is structurally related to regulatory factorsX1, X2, X4, and X5. It is a transcriptional activator that can bindDNA as a monomer or as a heterodimer with other RFX family members.Two transcript variants encoding different isoforms have beendescribed for this gene, and at least one of the variants utilizesalternative polyadenylation signals.
Background Reference 1:
El Zein, L., et al. J. Cell. Sci. 122 (PT 17), 3180-3189 (2009) :
Background Reference 2:
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
Background Reference 3:
Maijgren, S., et al. Arch. Dermatol. Res. 295(11):482-489(2004)
Background Reference 4:
Nakayama, A., et al. J. Biol. Chem. 278(1):233-240(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-549

List Size:
400 ul

List Price:

$495.00

Datasheet
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