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43-250 (2ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-250 (2ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-250 (2ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RFX5 Antibody

CATALOG NUMBER: 43-250

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TGDKSSEPSTLSNE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3ug/ml.
Specificity:
Reported variants represent identical protein: NP_000440.1, NP_001020774.1
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RFX5
Additional Names:
RFX5, regulatory factor X, 5 (influences HLA class II expression), OTTHUMP00000196318, regulatory factor X, 5
Protein Accession Number:
NP_000440.1
Protein Gi Number:
71040090
Ncbi Gene Id Number:
5993
Background Reference 1:
Kong X, Fang M, Li P, Fang F, Xu Y, HDAC2 deacetylates class II transactivator and suppresses its activity in macrophages and smooth muscle cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-250

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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