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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

RFXAP Antibody

CATALOG NUMBER: 57-775

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RFXAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the C-terminal region of human RFXAP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RFXAP
Additional Names:
Regulatory factor X-associated protein, RFX-associated protein, RFX DNA-binding complex 36 kDa subunit, RFXAP
Protein Accession Number:
O00287
Ncbi Gene Id Number:
5994
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Major histocompatibility (MHC) class II molecules aretransmembrane proteins that have a central role in development andcontrol of the immune system. The protein encoded by this gene,along with regulatory factor X-associated ankyrin-containingprotein and regulatory factor-5, forms a complex that binds to theX box motif of certain MHC class II gene promoters and activatestheir transcription. Once bound to the promoter, this complexassociates with the non-DNA-binding factor MHC class IItransactivator, which controls the cell type specificity andinducibility of MHC class II gene expression. Mutations in thisgene have been linked to bare lymphocyte syndrome type II,complementation group D. Transcript variants utilizing differentpolyA signals have been found for this gene.
Background Reference 1:
Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
Background Reference 2:
Bushway, M., et al. Immunogenetics 62(2):109-116(2010)
Background Reference 3:
Briggs, L., et al. Proteins 76(3):655-664(2009)
Background Reference 4:
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-775

List Size:
400 ul

List Price:

$495.00

Datasheet
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