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Western blot analysis of RGP1 in human heart tissue lysate with RGP1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of RGP1 in human heart tissue lysate with RGP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RGP1 Antibody

CATALOG NUMBER: 4569

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
RGP1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human RGP1.
The immunogen is located within amino acids 50 - 100 of RGP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RGP1 antibody can be used for detection of RGP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
RGP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RGP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RGP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RGP1
Additional Names:
RGP1 Antibody: KIAA0258, KIAA0258, Retrograde Golgi transport protein RGP1 homolog
Protein Accession Number:
NP_001073965
Protein Gi Number:
122937426
Ncbi Gene Id Number:
9827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RGP1 Antibody: Retrograde golgi transport homolog 1 (RGP1) is the mammalian homolog to the yeast RGP1, a protein that forms a tight complex with RIC1. This complex binds Ypt6p and stimulates guanine nucleotide exchange. RGP1 is localized to the Golgi and is thought to be a potential Golgi recycling factor. Rgp1 yeast mutants exhibit defects in retrograde trafficking similar to those seen in yeast with mutations in other retrograde Golgi transport proteins. It is expected that RGP1 plays a similar role in mammalian cells to that seen in yeast.
Background Reference 1:
Siniossoglou S, Peak-Chew SY, and Pelham HRB. Ric1p and Rgp1 form a complex that catalyzes nucleotide exchange on Ypt6p. EMBO J.2000; 19:4885-94.
Background Reference 2:
Panek HR, Conibear E, Bryan JD, et al. Identification of Rgp1, a novel Golgi recycling factor, as a protein required for efficient localization of yeast casein kinase 1 to the plasma membrane. J. Cell Sci.113:4545-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4569

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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