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Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of RGS21 in HepG2 cells with RGS21 antibody at 2.5 μg/mL.
Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.
Immunofluorescence of RGS21 in HepG2 cells with RGS21 antibody at 20 μg/mL.<br><br>Green: RGS21 Antibody (5209) <br> Blue: DAPI staining
Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

RGS21 Antibody

CATALOG NUMBER: 5209

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21.
The immunogen is located within amino acids 40 - 90 of RGS21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RGS21 antibody can be used for detection of RGS21 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Purification:
RGS21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RGS21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RGS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RGS21
Additional Names:
RGS21 Antibody: Regulator of G-protein signaling 21, RGS21
Protein Accession Number:
NP_001034241
Protein Gi Number:
85540441
Ncbi Gene Id Number:
431704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RGS21 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.
Background Reference 1:
De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA1995; 92:11916-20.
Background Reference 2:
Berman DM, Wilkie TM, and Gilman AG. GAIP and RGS4 are GTP-ase activating proteins for the Gi subfamily of G protein alpha subunits. Cell1996; 86:445-52.
Background Reference 3:
von Bucholtz L, Elischer A, Tareilus E, et al. RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells. Eur. J. Neurosci.2004; 19:1535-44.
Background Reference 4:
Li X, Chen L, Ji C, et al. Isolation and expression pattern of RGS21 gene, a novel RGS member. Acta Biochim. Pol.2005; 52:943-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5209

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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