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<strong> WB Validation in A549 Cells </strong><br>Loading: 15 μg of cell lysate Antibodies: RHBDD1, 5525 1 μg/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min
<strong> WB Validation in Mouse Heart </strong><br>Loading: 15 μg of lysate Antibodies: RHBDD1, 5525 1 μg/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 5 min
WB Validation in A549 Cells
Loading: 15 μg of cell lysate Antibodies: RHBDD1, 5525 1 μg/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min
Trial Size

RHBDD1 Antibody

CATALOG NUMBER: 5525

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (86%)
Immunogen:
RHBDD1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1.
The immunogen is located within the last 50 amino acids of RHBDD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RHBDD1 antibody can be used for detection of RHBDD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
RHBDD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RHBDD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RHBDD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RHBDD1
Additional Names:
RHBDD1 Antibody: RRP4, RHBDL4, HSD-50, HSD50, Rhomboid-related protein 4, Rhomboid domain-containing protein 1, RRP4
Protein Accession Number:
AAI01265
Protein Gi Number:
71680537
Ncbi Gene Id Number:
84236
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RHBDD1 Antibody: The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.
Background Reference 1:
Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol.2003; 4:R19.
Background Reference 2:
Wang Y, Guan X, Fok KL, et al. A novel member of the rhomboid family, RHBDD1, regulates BIK-mediated apoptosis. Cell Mol. Life Sci.2008; 65:3822-9.
Background Reference 3:
Wang Y, Song W, Li S, et al. GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules. BNC Cell Biol.2009; 10:25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5525

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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