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Western blot analysis of RHBDD3 in rat lung tissue lysate with RHBDD3 antibody at 1 μg/mL.
Immunofluorescence of RHBDD32 in rat lung tissue with RHBDD3 antibody at 20 μg/mL.
Western blot analysis of RHBDD3 in rat lung tissue lysate with RHBDD3 antibody at 1 μg/mL.
Trial Size

RHBDD3 Antibody

CATALOG NUMBER: 5529

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RHBDD3 antibody was raised against a 13 amino acid synthetic peptide from near the center of human RHBDD3.
The immunogen is located within amino acids 170 - 220 of RHBDD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
RHBDD3 antibody can be used for detection of RHBDD3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Purification:
RHBDD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RHBDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RHBDD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RHBDD3
Additional Names:
RHBDD3 Antibody: PTAG, C22orf3, HS984G1A, Rhomboid domain-containing protein 3
Protein Accession Number:
NP_036397
Protein Gi Number:
11072101
Ncbi Gene Id Number:
25807
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RHBDD3 Antibody: The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD3 was initially identified as a novel pituitary tumor apoptosis gene (PTAG) whose expression was reduced in certain pituitary adenomas. Overexpression of RHBDD3 in AtT20 cells showed an increase in apoptotic activity and caspase activation in response to bromocriptine, and apoptosis-inducing dopamine D2 analog, suggesting that reactivation of RHBDD3 in tumors may be a therapeutically useful tool. Later experiments also showed that RHBDD3 expression is also reduced in several primary colorectal tumors, indicating that loss of RHBDD3 contributes to a blunted apoptotic response and probably predisposes cells towards malignant transformation.
Background Reference 1:
Koonin EV, Makarova KS, Rogozin IB, et al. The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple horizontal gene transfers. Genome Biol.2003; 4:R19.
Background Reference 2:
Wang Y, Guan X, Fok KL, et al. A novel member of the rhomboid family, RHBDD1, regulates BIK-mediated apoptosis. Cell Mol. Life Sci.2008; 65:3822-9.
Background Reference 3:
Bahar A, Whitby P, Holley S, et al. Primary colorectal tumors fail to express the proapoptotic mediator PTAG and its reexpression augments drug-induced apoptosis. Genes Chomosomes Cancer2007; 46:202-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5529

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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